中科大张华凤研究组使用Vazyme ACE® 在Cell Researh发表论文

中科大张华凤研究组使用Vazyme ACE® 在Cell Researh发表论文

发布日期:2015-06-05 浏览次数:1225

中科大张华凤研究组在Cell Researh上发表题为“cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions”的论文。




该文章中使用了Vazyme品牌的AceQ® qPCR SYBR® Green Master Mix,(货号Q111),体现了Vazyme的qPCR SYBR Green mix的严谨性和稳定性。


文献链接:http://www.nature.com/cr/journal/vaop/ncurrent/full/cr201533a.html


Linchong Sun, Libing Song, Qianfen Wan, Gongwei Wu, Xinghua Li, Yinghui Wang, Jin Wang, Zhaoji Liu, Xiuying Zhong, Xiaoping He, Shengqi Shen, Xin Pan, Ailing Li, Yulan Wang, Ping Gao, Huiru Tang and Huafeng ZhangAbstractCancer cells are known to undergo metabolic reprogramming to sustain survival and rapid proliferation, however, it remains to be fully elucidated how oncogenic lesions coordinate the metabolic switch under various stressed conditions. Here we show that deprivation of glucose or glutamine, two major nutrition sources for cancer cells, dramatically activated serine biosynthesis pathway (SSP) that was accompanied by elevated cMyc expression. We further identified that cMyc stimulated SSP activation by transcriptionally upregulating expression of multiple SSP enzymes. Moreover, we demonstrated that SSP activation facilitated by cMyc led to elevated glutathione (GSH) production, cell cycle progression and nucleic acid synthesis, which are essential for cell survival and proliferation especially under nutrient-deprived conditions. We further uncovered that phosphoserine phosphatase (PSPH), the final rate-limiting enzyme of the SSP pathway, is critical for cMyc-driven cancer progression both in vitro and in vivo, and importantly, aberrant expression of PSPH is highly correlated with mortality in hepatocellular carcinoma (HCC) patients, suggesting a potential causal relation between this cMyc-regulated enzyme, or SSP activation in general, and cancer development. Taken together, our results reveal that aberrant expression of cMyc leads to the enhanced SSP activation, an essential part of metabolic switch, to facilitate cancer progression under nutrient-deprived conditions.